High-definition imaging using line-illumination modulation microscopy
Authors: Qiuyuan Zhong*, Anan Li*, Rui Jin, Dejie Zhang, Xiangning Li, Xueyan Jia, Pan Luo, Can Zhou, Chenyu Jiang, Zhao Feng, Zhihong Zhang, Hui Gong, Jing Yuan**, Qingming Luo**
* These authors contributed equally.
** Correspondence to: yuanj@hust.edu.cn; qluo@hust.edu.cn.

We report a novel LiMo technique to achieve excellent background removement in thick tissues with high throughput. Combining LiMo with thin tissue sectioning, we further developed a HD-fMOST method. We used HD-fMOST to image a resin-embedded whole mouse brain injected with AAV in the motor cortex at a voxel resolution of 0.32 × 0.32 × 1.00 µm over 111 hours.

The maximal-intensity projection (MIP) by using the whole-brain dataset of 12,678 coronal images:
Original coronal (100µm / 500µm / 1000µm)
Reconstructed sagittal (100µm / 500µm / 1000µm)
Reconstructed horizontal (100µm / 500µm / 1000µm)

Morphological reconstructions of 18 neurons.
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Acquisition software
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